A method to assess glyphosate, glufosinate and aminomethylphosphonic acid in soil and earthworms

A new sensitive and selective analytical methodology to quantify glyphosate (GLY), aminomethylphosphonic acid (AMPA), glufosinate (GLU) in both soil earthworms (Allolobophora chlorotica) was developed. The extraction purification methods were optimized. samples extracted with various aqueous solutions (HNO3, H2O, KOH borate buffer) derivatized 9-Fluorenylmethyl chloroformate (FMOCCl). To optimize the step, a method remove excess FMOCCl applied based on liquid-liquid diethyl ether. of extracts carried out using XLB solid phase (SPE) cartridges before internal standard quantification by liquid chromatography coupled tandem mass spectrometry (LC/MS/MS). elution step optimized obtain best recoveries possible, which acidic methanol (1% formic acid) (67% for GLY, 70% GLU 65% AMPA). followed analysis two herbicides AMPA soils LC/MS/MS determined limit (LOQ) values 0.030 ?g g ? 1 0.025 0.020 µg . For earthworms, LOQ 0.23 0.20 0.12 GLU. developed determine these compounds natural earthworms.